Artículos de revistas
Design of a slow cooling device for cryopreservation of small biological samples
Fecha
2015-12Registro en:
Juan de Paz, Leonardo; Robert, María Celeste; Graf, Daniel Adolfo; Guibert, Edgardo Elvio; Rodriguez, Joaquin Valentin; Design of a slow cooling device for cryopreservation of small biological samples; Royal Veterinary College; CryoLetters; 36; 12-2015; 363-371
0143-2044
CONICET Digital
CONICET
Autor
Juan de Paz, Leonardo
Robert, María Celeste
Graf, Daniel Adolfo
Guibert, Edgardo Elvio
Rodriguez, Joaquin Valentin
Resumen
BACKGROUND: Slow cooling is a cryopreservation methodology where samples are cooled to its storage temperature at controlled cooling rates. OBJECTIVE: Design, construction and evaluation of a simple and low cost device for slow cooling of small biological samples. MATERIALS AND METHODS: The device was constructed based on Pye?s freezer idea. A Dewar flask filled with liquid nitrogen was used as heat sink and a methanol bath containing the sample was cooled at constant rates using copper bars as heat conductor. RESULTS: Sample temperature may be lowered at controlled cooling rate (ranging from 0.4 ºC/min to 6.0 ºC/min) down to ~-60 ºC, where it could be conserved at lower temperatures. An example involving the cryopreservation of Neuro-2A cell line showed a marked influence of cooling rate over post preservation cell viability with optimal values between 2.6 and 4.6 °C/min. CONCLUSION: The cooling device proved to be a valuable alternative to more expensive systems allowing the assessment of different cooling rates to evaluate the optimal condition for cryopreservation of such samples.