Artículos de revistas
A model-independent algorithm to derive Ca2+ fluxes underlying local cytosolic Ca2+ transients
Fecha
2005-12Registro en:
Ventura, Alejandra; Bruno, Luciana; Demuro, Angelo; Parker, Ian; Ponce Dawson, Silvina Martha; A model-independent algorithm to derive Ca2+ fluxes underlying local cytosolic Ca2+ transients; Cell Press; Biophysical Journal; 88; 4; 12-2005; 2403-2421
0006-3495
CONICET Digital
CONICET
Autor
Ventura, Alejandra
Bruno, Luciana
Demuro, Angelo
Parker, Ian
Ponce Dawson, Silvina Martha
Resumen
Local intracellular Ca2+ signals result from Ca2+ flux into the cytosol through individual channels or clusters of channels. To gain a mechanistic understanding of these events we need to know the magnitude and spatial distribution of the underlying Ca2+ flux. However, this is difficult to infer from fluorescence Ca2+ images because the distribution of Ca2+-bound dye is affected by poorly characterized processes including diffusion of Ca2+ ions, their binding to mobile and immobile buffers, and sequestration by Ca2+ pumps. Several methods have previously been proposed to derive Ca2+ flux from fluorescence images, but all require explicit knowledge or assumptions regarding these processes. We now present a novel algorithm that requires few assumptions and is largely model-independent. By testing the algorithm with both numerically generated image data and experimental images of sparklets resulting from Ca2+ flux through individual voltage-gated channels, we show that it satisfactorily reconstructs the magnitude and time course of the underlying Ca2+ currents. © 2005 by the Biophysical Society.