Artículos de revistas
In mammalian foetal testes, SOX9 regulates expression of its target genes by binding to genomic regions with conserved signatures
Fecha
2017-07Registro en:
Rahmoun, Massilva; Lavery, Rowena; Laurent-Chaballier, Sabine; Bellora, Nicolás; Philip, Gayle K.; et al.; In mammalian foetal testes, SOX9 regulates expression of its target genes by binding to genomic regions with conserved signatures; Oxford University Press; Nucleic Acids Research; 45; 12; 7-2017; 7191-7211
1362-4962
CONICET Digital
CONICET
Autor
Rahmoun, Massilva
Lavery, Rowena
Laurent-Chaballier, Sabine
Bellora, Nicolás
Philip, Gayle K.
Rossitto, Moïra
Symon, Aleisha
Pailhoux, Eric
Cammas, Florence
Chung, Jessica
Bagheri-Fam, Stefan
Murphy, Mark
Bardwell, Vivian
Zarkower, David
Boizet Bonhoure, Brigitte
Clair, Philippe
Harley, Vincent R.
Poulat, Francis
Resumen
In mammalian embryonic gonads, SOX9 is required for the determination of Sertoli cells that orchestrate testis morphogenesis. To identify genetic networks directly regulated by SOX9, we combined analysis of SOX9-bound chromatin regions from murine and bovine foetal testes with sequencing of RNA samples from mouse testes lacking Sox9. We found that SOX9 controls a conserved genetic programme that involves most of the sex-determining genes. In foetal testes, SOX9 modulates both transcription and directly or indirectly sex-specific differential splicing of its target genes through binding to genomic regions with sequence motifs that are conserved among mammals and that we called 'Sertoli Cell Signature' (SCS). The SCS is characterized by a precise organization of binding motifs for the Sertoli cell reprogramming factors SOX9, GATA4 and DMRT1. As SOX9 biological role in mammalian gonads is to determine Sertoli cells, we correlated this genomic signature with the presence of SOX9 on chromatin in foetal testes, therefore equating this signature to a genomic bar code of the fate of foetal Sertoli cells. Starting from the hypothesis that nuclear factors that bind to genomic regions with SCS could functionally interact with SOX9, we identified TRIM28 as a new SOX9 partner in foetal testes.