Artículos de revistas
The importance of 2D aggregates on the antimicrobial resistance of Staphylococcus aureus sessile bacteria
Fecha
2016-04Registro en:
Miñan, Alejandro Guillermo; Schilardi, Patricia Laura; Fernandez Lorenzo, Monica Alicia; The importance of 2D aggregates on the antimicrobial resistance of Staphylococcus aureus sessile bacteria; Elsevier Science; Materials Science and Engineering: C; 61; 4-2016; 199-206
0928-4931
CONICET Digital
CONICET
Autor
Miñan, Alejandro Guillermo
Schilardi, Patricia Laura
Fernandez Lorenzo, Monica Alicia
Resumen
Biofilms formed on implanted devices are difficult to eradicate. Adhesion mechanism, high bacterial density, aggregation, induction of persisters and stressed bacteria are some of the factors considered when the antimicrobial resistance of these biofilms is analyzed. The aim of this work was to provide an alternative approach to the understanding of this issue by using a specially designed experimental set up that includes the use of microstructured (MS) surfaces (potential inhibitors of bacterial aggregation) in combination with antimicrobial agents (streptomycin and levofloxacin) against Staphylococcus aureus attached cells. Biofilms formed on smooth surfaces were used as plain controls (biofilmed-PC) characterized by the formation of dense 2D bacterial aggregates. Results showed bacterial persistence when streptomycin or levofloxacin were applied to PC-biofilms. The antimicrobial activity of both antibiotics was enhanced when bacteria were attached on MS, where single cells or small aggregates were observed. Thus, dense 2D aggregates of bacteria seem to be crucial as a required previous stage to develop the antimicrobial resistance.