Artículos de revistas
Transcriptional response of bovine monocyte-derived macrophages after the infection with different argentinean mycobacterium bovis Isolates
Fecha
2013-01Registro en:
Caimi, Karina Cynthia; Blanco, Federico Carlos; Soria, Marcelo Abel; Bigi, Fabiana; Transcriptional response of bovine monocyte-derived macrophages after the infection with different argentinean mycobacterium bovis Isolates; Hindawi Publishing Corporation; Biomedical Research-india; 2013; 1-2013; 1-9; 458278
0970-938X
CONICET Digital
CONICET
Autor
Caimi, Karina Cynthia
Blanco, Federico Carlos
Soria, Marcelo Abel
Bigi, Fabiana
Resumen
Infection of bovines with Mycobacterium bovis causes important financial hardship in many countries presenting also a risk for humans. M. bovis is known to be adapted to survive and thrive within the intramacrophage environment. In spite of its relevance, at present the information about macrophage expression patterns is scarce, particularly regarding the bovine host. In this study, transcriptomic analysis was used to detect genes differentially expressed in macrophages derived from peripheral blood mononuclear cells at early stages of infection with two Argentinean strains of M. bovis, a virulent and an attenuated strains. The results showed that the number of differentially expressed genes in the cells infected with the virulent strain (5) was significantly lower than those in the cells infected with the attenuated strain (172). Several genes were more strongly expressed in infected macrophages. Among them, we detected encoding transcription factors, anthrax toxin receptor, cell division and apoptosis regulator, ankyrin proteins, cytoskeleton proteins, protein of cell differentiation, and regulators of endocytic traffic of membrane. Quantitative real-time PCR of a selected group of differentially expressed genes confirmed the microarrays results. Altogether, the present results contribute to understanding the mechanisms involved in the early interaction of M. bovis with the bovine macrophage.