info:eu-repo/semantics/article
Two-Photon Optical Interrogation of Individual Dendritic Spines with Caged Dopamine
Fecha
2013-05Registro en:
Araya, Roberto; Andino Pavlovsky, Victoria; Yuste, Rafael; Etchenique, Roberto Argentino; Two-Photon Optical Interrogation of Individual Dendritic Spines with Caged Dopamine; American Chemical Society; ACS Chemical Neuroscience; 4; 8; 5-2013; 1163-1167
1948-7193
Autor
Araya, Roberto
Andino Pavlovsky, Victoria
Yuste, Rafael
Etchenique, Roberto Argentino
Resumen
We introduce a novel caged dopamine compound (RuBi-Dopa) based on ruthenium photochemistry. RuBi-Dopa has a high uncaging efficiency and can be released with visible (blue-green) and IR light in a two-photon regime. We combine two-photon photorelease of RuBi-Dopa with two-photon calcium imaging for an optical imaging and manipulation of dendritic spines in living brain slices, demonstrating that spines can express functional dopamine receptors. This novel compound allows mapping of functional dopamine receptors in living brain tissue with exquisite spatial resolution.