Artículos de revistas
Subchronic mycotoxicoses in Wistar rats: Assessment of the in vivo and in vitro genotoxicity induced by fumonisins and aflatoxin B1, and oxidative stress biomarkers status
Fecha
2010-12Registro en:
Theumer, Martín Gustavo; Cánepa, Mariana Clara; López, A. G.; Mary, Verónica Sofía; Dambolena, Jose Sebastian; et al.; Subchronic mycotoxicoses in Wistar rats: Assessment of the in vivo and in vitro genotoxicity induced by fumonisins and aflatoxin B1, and oxidative stress biomarkers status; Elsevier Ireland; Toxicology; 268; 1-2; 12-2010; 104-110
0300-483X
1879-3185
Autor
Theumer, Martín Gustavo
Cánepa, Mariana Clara
López, A. G.
Mary, Verónica Sofía
Dambolena, Jose Sebastian
Rubinstein, H. R.
Resumen
Some evidence suggests that fumonisin B1 (FB1), a worldwide toxic contaminant of grains produced by Fusarium verticillioides, exhibits an oxidative stress mediated genotoxicity. We studied the DNA damage (by the alkaline comet and the micronucleus tests) and biomarkers of cellular oxidative stress malondialdehyde, MDA; catalase, CAT; and superoxide dismutase, SOD) in spleen mononuclear cells of male Wistar rats subchronically (90 days) fed on a control experimental diet (CED) or poisoned with experimental diets contaminated with a culture material containing 100ppm of FB1 (FED), with 40 ppb of aflatoxin B1 (a common toxic co-contaminant in cereals, AFB1ED), and with a mixture of both toxins (MED). The DNA damage was found in 13.7%, 81.7%, 98.0% and 99.3% (comet assay) and in 2.8%, 7.0%, 10.8% and 8.8% (micronucleus technique) in groups CED, FED, AFB1ED and MED, respectively. The MDA levels as well as the CAT and SOD activities were increased in all the poisoned animals. A similar behavior was observed in cells exposed in vitro to the toxins. These data support the hypothesis of an oxidative stress mediated genotoxicity induced by FB1. Furthermore, the extent of DNA damage assessed by the comet assay suggests a possible protective effect of the fumonisins?AFB1 mixtures in vitro against the genotoxicity induced individually by the toxins.