Artículos de revistas
Depletion of the SR-related protein TbRRM1 leads to cell cycle arrest and apoptosis-like death in Trypanosoma brucei
Fecha
2015-08Registro en:
Levy, Gabriela Vanesa; Bañuelos, Carolina Paula; Níttolo, Analía Gabriela; Ortiz, Gastón Ezequiel; Mendiondo, Nicolas; et al.; Depletion of the SR-related protein TbRRM1 leads to cell cycle arrest and apoptosis-like death in Trypanosoma brucei; Public Library of Science; Plos One; 10; 8; 8-2015; 1-20
1932-6203
CONICET Digital
CONICET
Autor
Levy, Gabriela Vanesa
Bañuelos, Carolina Paula
Níttolo, Analía Gabriela
Ortiz, Gastón Ezequiel
Mendiondo, Nicolas
Moretti, Georgina
Tekiel, Valeria Sonia
Sanchez, Daniel Oscar
Resumen
Arginine-Serine (RS) domain-containing proteins are RNA binding proteins with multiple functions in RNA metabolism. In mammalian cells this group of proteins is also implicated in regulation and coordination of cell cycle and apoptosis. In trypanosomes, an early branching group within the eukaryotic lineage, this group of proteins is represented by 3 members, two of them are SR proteins and have been recently shown to be involved in rRNA processing as well as in pre-mRNA splicing and stability. Here we report our findings on the 3rd member, the SR-related protein TbRRM1. In the present study, we showed that TbRRM1 ablation by RNA-interference in T. brucei procyclic cells leads to cell-cycle block, abnormal cell elongation compatible with the nozzle phenotype and cell death by an apoptosis-like mechanism. Our results expand the role of the trypanosomal RS-domain containing proteins in key cellular processes such as cell cycle and apoptosis-like death, roles also carried out by the mammalian SR proteins, and thus suggesting a conserved function in this phylogenetically conserved protein family.