Artículos de revistas
Exopolysaccharides from Lactobacillus delbrueckii OLL1073R-1 modulate innate antiviral immune response in porcine intestinal epithelial cells
Fecha
2018-01Registro en:
Kanmani, Paulraj; Albarracín, Leonardo Miguel; Kobayashi, Hisakazu; Iida, Hikaru; Komatsu, Ryoya; et al.; Exopolysaccharides from Lactobacillus delbrueckii OLL1073R-1 modulate innate antiviral immune response in porcine intestinal epithelial cells; Pergamon-Elsevier Science Ltd; Molecular Immunology; 93; 1-2018; 253-265
0161-5890
1872-9142
CONICET Digital
CONICET
Autor
Kanmani, Paulraj
Albarracín, Leonardo Miguel
Kobayashi, Hisakazu
Iida, Hikaru
Komatsu, Ryoya
Kober, Humayun A. K. M.
Ikeda-Ohtsubo, Wakako
Suda, Yoshihito
Aso, Hisashi
Makino, Seiya
Kano, Hiroshi
Saito, Tadao
Villena, Julio Cesar
Kitazawa, Haruki
Resumen
Previous studies demonstrated that the extracellular polysaccharides (EPSs) produced by Lactobacillus delbrueckii OLL1073R-1 (LDR-1) improve antiviral immunity, especially in the systemic and respiratory compartments. However, it was not studied before whether those EPSs are able to beneficially modulate intestinal antiviral immunity. In addition, LDR-1-host interaction has been evaluated mainly with immune cells while its interaction with intestinal epithelial cells (IECs) was not addressed before. In this work, we investigated the capacity of EPSs from LDR-1 to modulate the response of porcine IECs (PIE cells) to the stimulation with the Toll-like receptor (TLR)-3 agonist poly(I:C) and the role of TLR2, TLR4, and TLR negative regulators in the immunoregulatory effect. We showed that innate immune response triggered by TLR3 activation in porcine IECs was differentially modulated by EPS from LDR-1. EPSs treatment induced an increment in the expression of interferon (IFN)-α and IFN-β in PIE cells after the stimulation with poly(I:C) as well as the expression of the antiviral factors MxA and RNase L. Those effects were related to the reduced expression of A20 in EPS-treated PIE cells. EPS from LDR-1 was also able to reduce the expression of IL-6 and proinflammatory chemokines. Although further in vivo studies are needed, our results suggest that these EPSs or a yogurt fermented with LDR-1 have potential to improve intestinal innate antiviral response and protect against intestinal viruses.