Artículos de revistas
Interaction of dopamine D1 with NMDA NR1 receptors in rat prefrontal cortex
Fecha
2009-01Registro en:
Kruse, Maria Sol; Prémont, Joel; Krebs, Marie Odile; Jay, Therese M.; Interaction of dopamine D1 with NMDA NR1 receptors in rat prefrontal cortex; Elsevier; European Neuropsychofarmacology; 19; 4; 1-2009; 296-304
0924-977X
1873-7862
CONICET Digital
CONICET
Autor
Kruse, Maria Sol
Prémont, Joel
Krebs, Marie Odile
Jay, Therese M.
Resumen
Despite the tremendous importance of D1 and NMDA receptors to cognition (working memory, executive functions) and synaptic plasticity in the prefrontal cortex (PFC), little is known about the molecular mechanisms underlying D1-NMDA receptors interactions in this brain area. Here, we show that D1 receptors and the NMDA receptor co-localize in single pyramidal neurons and interneurons in adult rat PFC. NR1 and NR2A expression are found in different neuronal types. Conversely, D1 receptors are predominantly localized in pyramidal-like cells and parvalbumin positive cells. NR1 co-immunoprecipitates with D1 receptor in adult medial PFC. In prefrontal primary cultures, NMDA does not affect the D1 receptor dependent-cAMP production. In contrast, activation of D1 receptor potentiates the NMDA mediated increase in cytosolic Ca2+, an effect that was blocked by a PKA inhibitor. We conclude that D1 receptor potentiates the NMDA-Ca2+ signal by a PKA-dependent mechanism.