info:eu-repo/semantics/article
N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes
Fecha
2011-10Registro en:
Calzadilla, Pablo Ignacio; Sapochnik, Daiana; Cosentino, María Soledad; Diz, Virginia Emilse; Dicelio, Lelia Elina; et al.; N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 12; 10; 10-2011; 6936-6951
1422-0067
1422-0067
Autor
Calzadilla, Pablo Ignacio
Sapochnik, Daiana
Cosentino, María Soledad
Diz, Virginia Emilse
Dicelio, Lelia Elina
Calvo, Juan Carlos
Guerra, Liliana Noemi
Resumen
Oxidative stress plays a critical role in the pathogenesis of diabetes, hypertension and atherosclerosis. Some authors reported that fat accumulation correlates to systemic oxidative stress in humans and mice, but the relationship of lipid production and oxidative metabolism is still unclear. In our laboratory we used 3T3-L1 preadipocytes, which are able to differentiate into mature adipocytes and accumulate lipids, as obesity model. We showed that intracellular reactive oxygen species (ROS) and antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities increased in parallel with fat accumulation. Meanwhile N-acetylcysteine (NAC), a well known antioxidant and Glutathione (GSH) precursor, inhibited ROS levels as well as fat accumulation in a concentration-dependent manner. NAC also inhibited both adipogenic transcription factors CCAAT/enhancer binding protein beta (C/EBP) and peroxisomal proliferator activated receptor gamma (PPAR β) expression; we suggested that intracellular GSH content could be responsible for these effects.