info:eu-repo/semantics/article
Identification and characterization of antioxidant peptides obtained by gastrointestinal digestion of amaranth proteins
Fecha
2016-04Registro en:
Orsini Delgado, María Cecilia; Nardo, Agustina Estefania; Pavlovic, Marija; Rogniaux, Hélène; Añon, Maria Cristina; et al.; Identification and characterization of antioxidant peptides obtained by gastrointestinal digestion of amaranth proteins; Elsevier; Food Chemistry; 197; 4-2016; 1160-1167
0308-8146
CONICET Digital
CONICET
Autor
Orsini Delgado, María Cecilia
Nardo, Agustina Estefania
Pavlovic, Marija
Rogniaux, Hélène
Añon, Maria Cristina
Tironi, Valeria Anahi
Resumen
The objective of the present work was to separate and identify antioxidant peptides from a simulated gastrointestinal digest (Id) from Amaranthus mantegazzianus proteins (I), which has previously been demonstrated to have this activity. I and Id were separated by preparative RP-HPLC. Fractions were evaluated by the ORAC method and the more active ones were analyzed by LC-MS/MS. Each fraction presented diverse peptides from different proteins, most of them from the 11S globulin. After grouping the peptides from 11S globulin according to their overlapping sequences, and based on previous information about structure-activity relationships, ten sequences were synthesized, in order to evaluate their antioxidant activity. Four peptides presented interesting activity: AWEEREQGSR > YLAGKPQQEH ∼ IYIEQGNGITGM ∼ TEVWDSNEQ. They exhibited some of the structural characteristics already known to demonstrate this activity, all of them containing at least one bulky aromatic residue. All belonged to little structured, internal or exposed regions of the acid subunit of the 11S globulin.