info:eu-repo/semantics/article
ChIP-seq analysis of the LuxR-type regulator VjbR reveals novel insights into the Brucella virulence gene expression network
Fecha
2017-03Registro en:
Kleinman, Claudia Laura; Sycz, Gabriela; Bonomi, Hernán Ruy; Rodríguez, Romina M.; Zorreguieta, Ángeles; et al.; ChIP-seq analysis of the LuxR-type regulator VjbR reveals novel insights into the Brucella virulence gene expression network; Oxford University Press; Nucleic Acids Research; 45; 10; 3-2017; 5757-5769
0305-1048
1362-4962
CONICET Digital
CONICET
Autor
Kleinman, Claudia Laura
Sycz, Gabriela
Bonomi, Hernán Ruy
Rodríguez, Romina M.
Zorreguieta, Ángeles
Sieira, Rodrigo
Resumen
LuxR-type transcription factors control diverse physiological functions necessary for bacterial adaptation to environmental changes. In the intracellular pathogen Brucella, the LuxR homolog VjbR has been shown to regulate the expression of virulence factors acting at early stages of the intracellular infection and, directly or indirectly, hundreds of additional genes. However, the precise determination of VjbR direct targets has so far proved elusive. Here, we performed chromatin immunoprecipitation of VjbR followed by next-generation sequencing (ChIP-seq). We detected a large amount of VjbR-binding sites distributed across the Brucella genome and determined a markedly asymmetric binding consensus motif, an unusual feature among LuxR-type regulators. RNA-seq analysis performed under conditions mimicking the eukaryotic intracellular environment revealed that, among all loci associated to VjbR-binding, this regulator directly modulated the expression of only a subset of genes encoding functions consistent with an intracellular adaptation strategy for survival during the initial stages of the host cell infection. Other VjbR-binding events, however, showed to be dissociated from transcription and may require different environmental signals to produce a transcriptional output. Taken together, our results bring new insights into the extent and functionality of LuxR-type-related transcriptional networks