Artículos de revistas
Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces
Fecha
2015-06Registro en:
Carnevale, Silvana; Pantano, Maria Laura; Kamenetzky, Laura; Malandrini, Jorge Bruno; Soria, Claudia Cecilia; et al.; Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces; De Gruyter; Acta Parasitologica; 60; 2; 6-2015; 211-217
1230-2821
CONICET Digital
CONICET
Autor
Carnevale, Silvana
Pantano, Maria Laura
Kamenetzky, Laura
Malandrini, Jorge Bruno
Soria, Claudia Cecilia
Velásquez, Jorge Néstor
Resumen
Fasciolosis is an important parasitic zoonosis considered the most important helminth infection of ruminants in tropical countries. The aim of this study was to develop a PCR assay for the sensitive and specific detection of F. hepatica in formalin preserved sheep faeces. A 405-bp fragment of the cytochrome c oxidase subunit 1 gene of F. hepatica was amplified from stool samples of infected sheep. The PCR assay showed a detection limit of 20 pg of F. hepatica DNA. No cross-reactions were observed with samples containing coccidian oocysts or gastrointestinal nematodes eggs. Our PCR technique showed to be effective for specific detection of F. hepatica infections in sheep.