Artículos de revistas
Depicting the discrepancy between Tri genotype and chemotype on the basis of strain CBS 139514 from a field population of F. graminearum sensu stricto from Argentina
Fecha
2016-11Registro en:
Kulik, Tomasz; Busko, Maciej; Bilska, Katarzyna; Ostrowska-Kołodziejczak, Anna; van Diepeningen, Anne D.; et al.; Depicting the discrepancy between Tri genotype and chemotype on the basis of strain CBS 139514 from a field population of F. graminearum sensu stricto from Argentina; Molecular Diversity Preservation International; Toxins; 8; 11; 11-2016; 330-337
2072-6651
CONICET Digital
CONICET
Autor
Kulik, Tomasz
Busko, Maciej
Bilska, Katarzyna
Ostrowska-Kołodziejczak, Anna
van Diepeningen, Anne D.
Perkowski, Juliusz
Stenglein, Sebastian Alberto
Resumen
Recent studies on a field population of F. graminearum sensu stricto from Argentina revealed an atypical panel of strains identified through PCR genotyping as 15ADON genotypes, but producing high levels of 3ADON. Based on representative strain CBS 139514, we asked if the discrepancy between trichothecene genotype and chemotype might result from inter-chemotype recombination of the chemotype-determining genes. To answer this, we sequenced the complete core Tri gene cluster (around 30,200 bp) from this strain and compared its sequence to sequence data of typical type B trichothecene genotypes/chemotypes. Sequence alignment showed that CBS 139514 has an identical sequence within the entire core Tri cluster to the 15ADON genotype. The revealed discrepancy underlines the need for using both molecular and chemical methods for reliable characterization of toxigenic strains of Fusarium.