Artículos de revistas
Vaccine strategies against Babesia bovis based on prime-boost immunizations in mice with modified vaccinia Ankara vector and recombinant proteins
Fecha
2014-06-23Registro en:
Jaramillo Ortiz, José Manuel; del Medico Zajac, Maria Paula; Zanetti, Flavia Adriana; Molinari, Maria Paula; Gravisaco, María José; et al.; Vaccine strategies against Babesia bovis based on prime-boost immunizations in mice with modified vaccinia Ankara vector and recombinant proteins; Elsevier; Vaccine; 32; 36; 23-6-2014; 4625-4632
0264-410X
Autor
Jaramillo Ortiz, José Manuel
del Medico Zajac, Maria Paula
Zanetti, Flavia Adriana
Molinari, Maria Paula
Gravisaco, María José
Calamante, Gabriela
Wilkowky, Silvina Elizabeth
Resumen
In this study, a recombinant modified vaccinia virus Ankara vector expressing a chimeric multi-antigenwas obtained and evaluated as a candidate vaccine in homologous and heterologous prime-boost immu-nizations with a recombinant protein cocktail. The chimeric multi-antigen comprises immunodominantB and T cell regions of three Babesia bovis proteins. Humoral and cellular immune responses were eval-uated in mice to compare the immunogenicity induced by different immunization schemes. The bestvaccination scheme was achieved with a prime of protein cocktail and a boost with the recombinantvirus. This scheme induced high level of specific IgG antibodies and secreted IFN and a high degree ofactivation of IFN+CD4+and CD8+specific T cells. This is the first report in which a novel vaccine can-didate was constructed based on a rationally designed multi-antigen and evaluated in a prime-boostregime, optimizing the immune response necessary for protection against bovine babesiosis.