PCR-based prediction of type I β-exotoxin production in Bacillus thuringiensis strains

dc.creatorSauka, Diego Herman
dc.creatorPerez, Melisa Paula
dc.creatorLopez, Nanci N.
dc.creatorOnco, María Inés
dc.creatorBerretta, Marcelo Facundo
dc.creatorBenintende, Graciela Beatriz
dc.date.accessioned2018-02-15T16:59:37Z
dc.date.accessioned2018-11-06T13:21:26Z
dc.date.available2018-02-15T16:59:37Z
dc.date.available2018-11-06T13:21:26Z
dc.date.created2018-02-15T16:59:37Z
dc.date.issued2014-10
dc.identifierSauka, Diego Herman; Perez, Melisa Paula; Lopez, Nanci N.; Onco, María Inés; Berretta, Marcelo Facundo; et al.; PCR-based prediction of type I β-exotoxin production in Bacillus thuringiensis strains; Academic Press Inc Elsevier Science; Journal of Invertebrate Pathology; 122; 10-2014; 28-31
dc.identifier0022-2011
dc.identifierhttp://hdl.handle.net/11336/36540
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1874657
dc.description.abstractSome Bacillus thuringiensis strains secrete type I β-exotoxin, which is a non-specific insecticidal and thermostable adenine nucleoside oligosaccharide. Toxicity bioassays and HPLC are traditional methods for detecting β-exotoxin. With the aim of establish a first rapid approach for prediction of type I β-exotoxin production, two PCR-based methods were successfully evaluated in B. thuringiensis strains and native isolates. In order to validate a reliable technology, results obtained by this method were correlated with that obtained from Musca domestica bioassays.
dc.languageeng
dc.publisherAcademic Press Inc Elsevier Science
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jip.2014.08.001
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S002220111400113X
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectBacillus thuringiensis
dc.subjectβ-exotoxin
dc.subjectthuE gene
dc.subjectPCR
dc.titlePCR-based prediction of type I β-exotoxin production in Bacillus thuringiensis strains
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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