Artículos de revistas
Antioxidant activity and cardioprotective effect of a nonalcoholic extract of Vaccinium meridionale Swartz during ischemia-reperfusion in rats
Fecha
2013-03Registro en:
Lopera, Yasmin E.; Fantinelli, Juliana Catalina; González Arbeláez, Luisa Fernanda; Rojano, Benjamín; Ríos, José Luis; et al.; Antioxidant activity and cardioprotective effect of a nonalcoholic extract of Vaccinium meridionale Swartz during ischemia-reperfusion in rats; Hindawi Publishing Corporation; Evidence-based Complementary And Alternative Medicine; 2013; 3-2013; 1-10; 516727
1741-427X
1741-4288
Autor
Lopera, Yasmin E.
Fantinelli, Juliana Catalina
González Arbeláez, Luisa Fernanda
Rojano, Benjamín
Ríos, José Luis
Schinella, Guillermo
Mosca, Susana Maria
Resumen
Our objective was to assess the antioxidant properties and the effects against the reperfusion injury of a nonalcoholic extract obtained by fermentation from the Colombian blueberry, mortino ( Vaccinium meridionale Swartz, Ericaceae). Antioxidant properties were assessed by in vitro systems. To examine the postischemic myocardial function, isolated rat hearts were treated 10 min before ischemia and during the first 10 min of reperfusion with the extract. To analyze the participation of nitric oxide (NO), other experiments were performed in the presence of nitric oxide synthase (NOS) inhibition with NG-nitro-L-arginine methyl ester (L-NAME). In cardiac tissue thiobarbituric acid reactive substances (TBARS) concentration, reduced glutathione (GSH) content, endothelial NOS (eNOS), and Akt expression were also measured. The blueberry extract showed higher total phenols and anthocyanins contents, scavenging activity of superoxide radical and systolic and diastolic function was improved, TBARS diminished, GSH was partially preserved, and both NOS and Akt expression increased in hearts treated with the extract. These beneficial effects were lost when eNOS was inhibited. In resume, these data show that the increase of eNOS expression via Akt and the scavenging activity contribute to the cardioprotection afforded by acute treatment with Colombian blueberry extract against ischemia and reperfusion injury.