Artículos de revistas
Isolation and characterization of 14 tetranucleotide microsatellite loci for the cannonball jellyfish (Stomolophus sp.) by next generation sequencing
Fecha
2017-04Registro en:
Getino Mamet, Leandro Nicolás; Valdivia Carrillo, Tania; Gómez Daglio, Liza ; García De León, Francisco Javier ; Isolation and characterization of 14 tetranucleotide microsatellite loci for the cannonball jellyfish (Stomolophus sp.) by next generation sequencing; Springer; Molecular Biology Reports; 44; 2; 4-2017; 257–260
0301-4851
1573-4978
CONICET Digital
CONICET
Autor
Getino Mamet, Leandro Nicolás
Valdivia Carrillo, Tania
Gómez Daglio, Liza
García De León, Francisco Javier
Resumen
The Cannonball jellyfish (Stomolophus sp.) is a species of jellyfish with high relevance in artisanal fishing. Studies of their populations do not extend beyond the morphological descriptions knowing that presents a great morphological variability. However, there are no genetic studies to determine the number of independent populations, so microsatellite markers become a suitable option. Since there are no species-specific microsatellite loci, in this paper, 14 new microsatellite loci are characterized. Microsatellite loci were isolated de novo through next generation sequencing, by two runs on Illumina MiSeq. A total of 506,771,269 base pair were obtained, from which 142,616 were microsatellite loci, and 1546 of them could design primers. We tested 14 primer pairs on 32 individuals from Bahía de La Paz, Gulf of California. We observed low genetic variation among loci (mean number of alleles per locus = 4.33, mean observed heterozygosity 0.381, mean expected heterozygosity 0.501). These loci are the first ones described for the species and will be helpful to carry out genetic diversity and population genetics studies.