Glycosyltransferase complexes improve glycolipid synthesis

dc.creatorSpessott, Waldo Andrés
dc.creatorCrespo, Pilar María
dc.creatorDaniotti, Jose Luis
dc.creatorMaccioni, Hugo Jose Fernando
dc.date.accessioned2018-08-09T18:21:28Z
dc.date.accessioned2018-11-06T12:52:50Z
dc.date.available2018-08-09T18:21:28Z
dc.date.available2018-11-06T12:52:50Z
dc.date.created2018-08-09T18:21:28Z
dc.date.issued2012-07
dc.identifierSpessott, Waldo Andrés; Crespo, Pilar María; Daniotti, Jose Luis; Maccioni, Hugo Jose Fernando; Glycosyltransferase complexes improve glycolipid synthesis; Elsevier Science; FEBS Letters; 586; 16; 7-2012; 2346-2350
dc.identifier0014-5793
dc.identifierhttp://hdl.handle.net/11336/54812
dc.identifier1873-3468
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1870784
dc.description.abstractThe synthesis of gangliosides GM3 and GD3 is carried out by the successive addition of sialic acid residues on lactosylceramide (LacCer) by the Golgi located sialyltransferases Sial-T1 and Sial-T2, respectively. CHO-K1 cells lack Sial-T2 and only express GM3. Here we show that the activity of Sial-T1 was near 2.5-fold higher in homogenates of CHO-K1 cells transfected to express Sial-T2 (CHO-K1 Sial-T2) than in untransfected cells. The appearance of Sial-T1 enzyme or gene transcription activators or the stabilization of the Sial-T1 protein were discarded as possible causes of the activation. Sial-T2 lacking the catalytic domain failed to promote Sial-T1 activation. Since Gal-T1, Sial-T1 and Sial-T2 form a multienzyme complex, we propose that transformation of formed GM3 into GD3 and GT3 by Sial-T2 in the complex leaves Sial-T1 unoccupied, enabled for new rounds of LacCer utilization, which results in its apparent activation. Crown Copyright © 2012 Published by Elsevier B.V. on behalf of Federation of European Biochemical society. All rights reserved.
dc.languageeng
dc.publisherElsevier Science
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://febs.onlinelibrary.wiley.com/doi/abs/10.1016/j.febslet.2012.05.041
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.febslet.2012.05.041
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectGLYCOLIPID SIALYLTRANSFERASES
dc.subjectGLYCOLIPID SYNTHESIS
dc.subjectGLYCOSYLTRANSFERASE MULTIENZYME COMPLEX
dc.subjectGM3 AND GD3 SYNTHESIS
dc.subjectGOLGI COMPLEX GLYCOSYLATION
dc.titleGlycosyltransferase complexes improve glycolipid synthesis
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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