Artículos de revistas
Histidine oxidation photosensitized by pterin: PH dependent mechanism
Fecha
2015-12Registro en:
Castaño Espinal, Diana Carolina; Oliveros, Esther; Thomas, Andrés Héctor; Lorente, Carolina; Histidine oxidation photosensitized by pterin: PH dependent mechanism; Elsevier Science Sa; Journal of Photochemistry and Photobiology B: Biology; 153; 12-2015; 483-489
1011-1344
CONICET Digital
CONICET
Autor
Castaño Espinal, Diana Carolina
Oliveros, Esther
Thomas, Andrés Héctor
Lorente, Carolina
Resumen
Aromatic pterins accumulate in the skin of patients suffering from vitiligo, a chronic depigmentation disorder, due to the oxidation of tetrahydrobiopterin, the biologically active form of pterins. In this work, we have investigated the ability of pterin, the parent compound of aromatic pterins, to photosensitize the oxidation of histidine in aqueous solutions under UV-A irradiation. Histidine is an α-amino acid with an imidazole functional group, and is frequently present at the active sites of enzymes. The results highlight the role of the pH in controlling the competition between energy and electron transfer mechanisms. It has been previously demonstrated that pterins participate as sensitizers in photosensitized oxidations, both by type I (electron-transfer) and type II mechanisms (singlet oxygen (1O2)). By combining different analytical techniques, we could establish that a type I photooxidation was the prevailing mechanism at acidic pH, although a type II mechanism is also present, but it is more important in alkaline solutions.