Artículos de revistas
Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?
Fecha
2013-01Registro en:
Pacheco, Maria Emilia; Bruzzone, Liliana; Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?; Elsevier Science; Journal of Luminescence; 137; 1-2013; 138-142
0022-2313
CONICET Digital
CONICET
Autor
Pacheco, Maria Emilia
Bruzzone, Liliana
Resumen
Synchronous fluorescence spectrometry has become a common tool to investigate conformational changes in proteins due to the presence of other molecules (quenchers). The same pattern is always followed, leaving aside the possibility of interfering effects that make conclusions incorrect. In this paper, we discuss the main cause of a wrong understanding of synchronous fluorescence spectrometry in a large number of papers published in many different journals. Based on the principles of synchronous fluorescence spectrometry, we perform a simple correction method of synchronous spectra taking into account inner filter effects. This criticism tries to demonstrate that the interpretation of synchronous fluorescence spectrometry is not as easy as it seems.