Artículos de revistas
Detection of class 1 and 2 integrons, extended-spectrum β-lactamases and qnr alleles in enterobacterial isolates from the digestive tract of Intensive Care Unit inpatients
Fecha
2010-11Registro en:
Bado, Inés; Cordeiro, Nicolás F.; Robino, Luciana; García Fulgueiras, Virginia; Seija, Verónica; et al.; Detection of class 1 and 2 integrons, extended-spectrum β-lactamases and qnr alleles in enterobacterial isolates from the digestive tract of Intensive Care Unit inpatients; Elsevier Science; International Journal Of Antimicrobial Agents; 36; 5; 11-2010; 453-458
0924-8579
Autor
Bado, Inés
Cordeiro, Nicolás F.
Robino, Luciana
García Fulgueiras, Virginia
Seija, Verónica
Bazet, Cristina
Gutkind, Gabriel Osvaldo
Ayala, Juan A.
Vignoli, Rafael
Resumen
In this study, we searched for extended-spectrum β-lactamases (ESBLs), class 1 and 2 integrons, and qnrA, qnrB and qnrS genes in 56 oxyimino-cephalosporin and/or ciprofloxacin-resistant enterobacterial isolates obtained from the gastrointestinal tract of patients admitted in an Intensive Care Unit in Uruguay. ESBLs were detected in 11 isolates (6 CTX-M-2, 3 CTX-M-9, 1 CTX-M-15 and 1 PER-2). qnr genes and integrons were detected in 5 and 24 isolates, respectively. Eight different antibiotic resistance gene cassettes were found within six different genetic arrangements. Two types of complex class 1 integrons carrying insertion sequence ISCR1 were found, one showing blaCTX-M-2–orf3 and the other qnrA1–ampR. Ten of the thirteen isolates carrying class 2 integrons presented the element IS5 inserted between intI2 and dfrA1, whereas another class 2 integron lacked the internal stop codon usually present in intI2. This is the first report of the occurrence of qnrA, qnrB and blaCTX-M-9 in Uruguay. Dissemination of the different groups of CTX-M enzymes (i.e. groups 1, 2 and 9) appears to be a recent phenomenon in South America.