The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells

dc.creatorLoreti, Nazareth
dc.creatorFresno, Cristobal
dc.creatorBarrera, David
dc.creatorAndreone, Luz
dc.creatorLira Albarran, Saúl
dc.creatorFernandez, Elmer Andres
dc.creatorLarrea, Fernando
dc.creatorCampo, Stella Maris
dc.date.accessioned2015-12-21T17:50:12Z
dc.date.accessioned2018-11-06T12:38:38Z
dc.date.available2015-12-21T17:50:12Z
dc.date.available2018-11-06T12:38:38Z
dc.date.created2015-12-21T17:50:12Z
dc.date.issued2013-12-05
dc.identifierLoreti, Nazareth; Fresno, Cristobal; Barrera, David; Andreone, Luz; Lira Albarran, Saúl; et al.; The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells; Elsevier; Molecular and Cellular Endocrinology; 366; 1; 5-12-2013; 68-80
dc.identifier0303-7207
dc.identifierhttp://hdl.handle.net/11336/3108
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1868627
dc.description.abstractThe aim of this study was to analyse the biological response to different recombinant human FSH (rhFSH)glycosylation variants on the endocrine activity and gene expression at whole-genome scale in human granulosa-like tumor cell line, KGN. The effects of differences in rhFSH sialylation and oligosaccharide complexity were determined on steroid hormone and inhibin production. A microarray approach was used to explore gene expression patterns induced by rhFSH glycosylation variants. Set enrichment analysis revealed that hormone sialylation and oligosaccharide complexity in rhFSH differentially affected the expression of genes involved in essential biological processes and molecular functions of KGN cells. The relevance of rhFSH oligosaccharide structure on teroidogenesis was confirmed assessing gene expression by real time-PCR. The results demonstrate that FSH oligosaccharide structure affects expression of genes encoding proteins, growth factors and hormones essential for granulosa cells function.
dc.languageeng
dc.publisherElsevier
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S030372071200528X
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.mce.2012.11.021
dc.relationinfo:eu-repo/semantics/altIdentifier/issn/0303-7207
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectFSH
dc.subjectGLYCOSYLATION VARIANT
dc.subjectSTEROID
dc.subjectINHIBIN
dc.subjectGENE EXPRESSION
dc.subjectHUMAN GRANULOSA CELL
dc.titleThe glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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