Artículos de revistas
The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells
Fecha
2013-12-05Registro en:
Loreti, Nazareth; Fresno, Cristobal; Barrera, David; Andreone, Luz; Lira Albarran, Saúl; et al.; The glycan structure in recombinant human FSH affects endocrine activity and global gene expression in human granulosa cells; Elsevier; Molecular and Cellular Endocrinology; 366; 1; 5-12-2013; 68-80
0303-7207
Autor
Loreti, Nazareth
Fresno, Cristobal
Barrera, David
Andreone, Luz
Lira Albarran, Saúl
Fernandez, Elmer Andres
Larrea, Fernando
Campo, Stella Maris
Resumen
The aim of this study was to analyse the biological response to different recombinant human FSH (rhFSH)glycosylation variants on the endocrine activity and gene expression at whole-genome scale in human granulosa-like tumor cell line, KGN. The effects of differences in rhFSH sialylation and oligosaccharide complexity were determined on steroid hormone and inhibin production. A microarray approach was used to explore gene expression patterns induced by rhFSH glycosylation variants. Set enrichment analysis revealed that hormone sialylation and oligosaccharide complexity in rhFSH differentially affected the expression of genes involved in essential biological processes and molecular functions of KGN cells. The relevance of rhFSH oligosaccharide structure on teroidogenesis was confirmed assessing gene expression by real time-PCR. The results demonstrate that FSH oligosaccharide structure affects expression of genes encoding proteins, growth factors and hormones essential for granulosa cells function.