Artículos de revistas
Water flux through human aquaporin 1: inhibition by intracellular furosemide and maximal response with high osmotic gradients
Fecha
2011-06Registro en:
Ozu, Marcelo; Dorr, Ricardo Alfredo; Politi, Teresa; Parisi, Mario Nestor; Toriano, Roxana Mabel; Water flux through human aquaporin 1: inhibition by intracellular furosemide and maximal response with high osmotic gradients; Springer; European Biophysics Journal With Biophysics Letters; 40; 6; 6-2011; 737-746
0175-7571
1432-1017
Autor
Ozu, Marcelo
Dorr, Ricardo Alfredo
Politi, Teresa
Parisi, Mario Nestor
Toriano, Roxana Mabel
Resumen
This work studies water permeability properties of human aquaporin 1 (hAQP1) expressed in Xenopus laevis oocyte membranes, applying a technique where cellular content is replaced with a known medium, with the possibility of measuring intracellular pressure. Consequences on water transport—produced by well-known anisotonic gradients and by the intracellular effect of probable aquaporin inhibitors—were tested. In this way, the specific intracellular inhibition of hAQP1 by the diuretic drug furosemide was demonstrated. In addition, experiments imposing anisotonic mannitol gradients with a constant ionic strength showed that the relationship between water flux and the applied mannitol gradient deflects from a perfect osmometer response when the gradient is higher than 150 mosmol kgW−1. These results would indicate that the passage of water molecules through hAQP1 may have a maximum rate. As a whole, this work demonstrates the technical advantage of controlling both intracellular pressure and medium composition in order to study biophysical properties of hAQP1, and contributes information on water channel behavior under osmotic challenges and the discovery of new inhibitors.