Artículos de revistas
Differential β₂-adrenergic receptor expression defines the phenotype of non-tumorigenic and malignant human breast cell lines
Fecha
2014-11-07Registro en:
Gargiulo, Lucía; Copsel, Sabrina Natalia; Rivero, Ezequiel Mariano; Galés, Céline; Sénard, Jean Michel; et al.; Differential β₂-adrenergic receptor expression defines the phenotype of non-tumorigenic and malignant human breast cell lines; Impact Journals; Oncotarget; 5; 20; 7-11-2014; 10058-10069
1949-2553
1949-2553
CONICET Digital
CONICET
Autor
Gargiulo, Lucía
Copsel, Sabrina Natalia
Rivero, Ezequiel Mariano
Galés, Céline
Sénard, Jean Michel
Luthy, Isabel Alicia
Davio, Carlos Alberto
Bruzzone, Ariana
Resumen
Breast cancer is the most frequent malignancy in women. Several reports demonstrated that adrenergic receptors (ARs) are involved in breast cancer. Here we observed that epinephrine (Epi), an endogenous AR agonist, caused opposite effects in non-tumorigenic (MCF-10A and HBL-100) and tumor cells (MCF-7 and MDA-MB-231). Thus, Epi, in non-tumor breast cells, as well as isoproterenol (β-agonist), in all cell lines, maintained a benign phenotype, decreasing cell proliferation and migration, and stimulating cell adhesion. β-AR expression and cAMP levels were higher in MCF-10A than in MCF-7 cells. β₂-AR knock-down caused a significant increase of cell proliferation and migration, and a decrease of cell adhesion both in basal and in Iso-stimulated conditions. Coincidently, β₂-AR over-expression induced a significant decrease of cell proliferation and migration, and an increase of cell adhesion. Therefore, β₂-AR is implied in cell phenotype and its agonists or antagonists could eventually complement cancer therapy.