info:eu-repo/semantics/article
Ethanol downregulates N- acyl phosphatidylethanolamine-phospholipase D expression in BV2 microglial cells via epigenetic mechanisms
Fecha
2016-09Registro en:
Correa, Fernando Gabriel; de Laurentiis, Andrea; Franchi, Ana Maria; Ethanol downregulates N- acyl phosphatidylethanolamine-phospholipase D expression in BV2 microglial cells via epigenetic mechanisms; Elsevier Science; European Journal of Pharmacology; 786; 9-2016; 224-233
0014-2999
CONICET Digital
CONICET
Autor
Correa, Fernando Gabriel
de Laurentiis, Andrea
Franchi, Ana Maria
Resumen
Excessive ethanol drinking has deleterious effects on the brain. However, the effects of alcohol on microglia, the main mediator of the brain´s innate immune response remain poorly understood. On the other hand, the endocannabinoid system plays a fundamental role in regulating microglial reactivity and function. Here we studied the effects of acute ethanol exposure to murine BV2 microglial cells on N-acyl phosphatidylethanolamine-phospholipase D (NAPE-PLD), a major synthesizing enzyme of anandamide and other N-acylethanolamines. We found that ethanol downregulated microglial NAPE-PLD expression by activating cAMP/PKA and ERK1/2. These signaling pathways converged on increased phosphorylation of CREB. Moreover, ethanol induced an increase in histone acetyltransferase activity which led to higher levels of acetylation of histone H3. Taken together, our results suggest that ethanol actions on microglial NAPE-PLD expression might involve epigenetic mechanisms.