Artículos de revistas
The role of cytoskeleton networks on lipid-mediated delivery of DNA
Fecha
2013-02Registro en:
Coppola, Stefano; Cardarelli, Francesco; Pozzi, Daniela; Estrada, Laura Cecilia; Digman, Michelle; et al.; The role of cytoskeleton networks on lipid-mediated delivery of DNA; Future Science; Therapeutic Delivery; 4; 2; 2-2013; 191-202
2041-5990
Autor
Coppola, Stefano
Cardarelli, Francesco
Pozzi, Daniela
Estrada, Laura Cecilia
Digman, Michelle
Gratton, Enrico
Bifone, Angelo
Marianecci, Carlotta
Caracciolo, Giulio
Resumen
Background: Lipid-mediated delivery of DNA is hindered by extracellular and intracellular barriers that significantly reduce the transfection efficiency of synthetic nonviral vectors. Results: In this study we investigated the role of the actin and microtubule networks on the uptake and cytoplasmic transport of multicomponent cationic liposome–DNA complexes in CHO-K1 live cells by means of confocal laser scanning microscopy and 3D single particle tracking. Treatment with actin (latrunculin B)- and microtubule-disrupting (nocodazole) reagents indicated that intracellular trafficking of complexes predominantly involves microtubule-dependent active transport. We found that the actin network has a major effect on the initial uptake of complexes, while the microtubule network is mainly responsible for the subsequent active transportation to the lysosomes. Conclusion: Collectively, a strategy to improve the efficiency of lipid gene vectors can be formulated. We could find a lipid formulation that allows the nanoparticles to avoid the microtubule pathway to lysosomes.