Artículos de revistas
A new colorimetric method for determination of alkylresorcinols in ground and whole-cereal grains using the diazonium salt Fast Blue RR
Fecha
2009-08Registro en:
Sampietro, Diego Alejandro; Vattuone, Marta Amelia; Catalan, Cesar Atilio Nazareno; A new colorimetric method for determination of alkylresorcinols in ground and whole-cereal grains using the diazonium salt Fast Blue RR; Elsevier; Food Chemistry; 115; 3; 8-2009; 1170-1174
0308-8146
CONICET Digital
CONICET
Autor
Sampietro, Diego Alejandro
Vattuone, Marta Amelia
Catalan, Cesar Atilio Nazareno
Resumen
A fast and inexpensive method was developed to determine the content of alkylresorcinols (ARs) in ground and whole-cereal grains. This method is based on the ability of ARs to couple with Fast Blue RR salt in alkaline medium, yielding coloured azo-derivatives that can be quantified colorimetrically. Good linearity was observed for olivetol in the range of 1-10 μg with methanol as solvent (λmax = 480 nm) and 1-7 μg with butanol as solvent (λmax = 530 nm). Sensitivity obtained in butanol was comparable to that obtained in the Fast Blue B based method (methanol as solvent, λmax = 520 nm). In the new colorimetric method described here, incubation time was reduced to 20 min and the stability of the reaction products was as long as 3 h. The method appears promising for the analysis of 1,3-dihydroxybenzene derivatives in samples from plant breeding and food analyses.