info:eu-repo/semantics/article
Continuous flow generation of magnetoliposomes in a low-cost portable microfluidic platform
Fecha
2014-09Registro en:
Conde, Alvaro José; Battalla, Milena; Cerda, Maria Belen; Mykhaylyk, Olga; Plank, Christian; et al.; Continuous flow generation of magnetoliposomes in a low-cost portable microfluidic platform; Royal Society Of Chemistry; Lab On A Chip; 14; 23; 9-2014; 4506-4512
1473-0197
Autor
Conde, Alvaro José
Battalla, Milena
Cerda, Maria Belen
Mykhaylyk, Olga
Plank, Christian
Podhajcer, Osvaldo Luis
Cabaleiro, Juan Martin
Madrid, Rossana Elena
Policastro, Lucia Laura
Resumen
We present a low-cost, portable microfluidic platform that uses laminated polymethylmethacrylate chips, peristaltic micropumps and LEGO® Mindstorms components for the generation of magnetoliposomes that does not require extrusion steps. Mixtures of lipids reconstituted in ethanol and an aqueous phase were injected independently in order to generate a combination of laminar flows in such a way that we could effectively achieve four hydrodynamic focused nanovesicle generation streams. Monodisperse magnetoliposomes with characteristics comparable to those obtained by traditional methods have been obtained. The magnetoliposomes are responsive to external magnetic field gradients, a result that suggests that the nanovesicles can be used in research and applications in nanomedicine.