An optimized DNA extraction protocol for benthic Didymosphenia geminata

Uyua, Noelia Mariel; Manrique, Julieta Marina; Jones, Leandro Roberto

Artículos de revistas

Fecha

2014-06

Registro en:

Uyua, Noelia Mariel; Manrique, Julieta Marina; Jones, Leandro Roberto; An optimized DNA extraction protocol for benthic Didymosphenia geminata; Elsevier Science; Journal Of Microbiological Methods; 104; 6-2014; 12-18

0167-7012

http://hdl.handle.net/11336/35693

CONICET Digital

CONICET

http://repositorioslatinoamericanos.uchile.cl/handle/2250/1862640

Autor

Uyua, Noelia Mariel

Manrique, Julieta Marina

Jones, Leandro Roberto

Institución

Consejo Nacional de Investigaciones Científicas y Tecnológicas (Argentina)

Resumen

Didymosphenia geminata mats display few cells in relation to extracellular material and contain polysaccharides and heavy metals that interfere with molecular studies. We describe an optimized DNA extraction protocol that help to overcome these difficulties. Our protocol outperformed five previously described DNA extraction techniques.

Materias

Didymosphenia

DNA extraction

PCR

Stalk

Benthic

Invasive

Mostrar el registro completo del ítem