Artículos de revistas
Intracellular Chloride Concentration Changes Modulate IL-1β Expression and Secretion in Human Bronchial Epithelial Cultured Cells
Fecha
2017-08Registro en:
Clauzure, Mariangeles; Valdivieso, Ángel Gabriel; Massip Copiz, María Macarena; Mori, Consuelo; Dugour, Andrea Vanesa; et al.; Intracellular Chloride Concentration Changes Modulate IL-1β Expression and Secretion in Human Bronchial Epithelial Cultured Cells; Wiley-liss, Div John Wiley & Sons Inc; Journal of Cellular Biochemistry; 118; 8; 8-2017; 2131-2140
0730-2312
CONICET Digital
CONICET
Autor
Clauzure, Mariangeles
Valdivieso, Ángel Gabriel
Massip Copiz, María Macarena
Mori, Consuelo
Dugour, Andrea Vanesa
Figueroa, Juan Manuel
Santa Coloma, Tomás Antonio
Resumen
Cystic fibrosis (CF) is caused by mutations in the CFTR gene, which encodes a cAMP‐regulated chloride channel. Several cellular functions are altered in CF cells. However, it is not clear how the CFTR failure induces those alterations. We have found previously several genes differentially expressed in CF cells, including c‐Src, MUC1, MTND4, and CISD1 (CFTR‐dependent genes). Recently, we also reported the existence of several chloride‐dependent genes, among them GLRX5 and RPS27. Here, varying the intracellular chloride concentration [Cl−]i of IB3‐1 CF bronchial epithelial cells, we show that IL‐1β mRNA expression and secretion are also under Cl− modulation. The response to Cl− is biphasic, with maximal effects at 75 mM Cl−. The regulation of the IL‐1β mRNA expression involves an IL‐1β autocrine effect, since in the presence of the IL‐1β receptor antagonist IL1RN or anti‐IL‐1β blocking antibody, the mRNA response to Cl− disappeared. Similar effects were obtained with the JNK inhibitor SP600125, the c‐Src inhibitor PP2 and the IKK inhibitor III (BMS‐345541). On the other hand, the IL‐1β secretion is still modulated by Cl− in the presence of IL‐1RN, IL‐1β blocking antibody, or cycloheximide, suggesting that Cl− is affecting the IL‐1β maturation/secretion, which in turn starts an autocrine positive feedback loop. In conclusion, the Cl− anion acts as a second messenger for CFTR, modulating the IL‐1β maturation/secretion. The results also imply that, depending on its intracellular concentration, Cl− could be a pro‐inflammatory mediator.