Artículos de revistas
Electrochemical sensing of guanine, adenine and 8-hydroxy-2′-deoxyguanosine at glassy carbon modified with single-walled carbon nanotubes covalently functionalized with lysine
Fecha
2016-01-21Registro en:
Gutierrez, Alejandro; Gutierrez, Fabiana Andrea; Eguílaz Rubio, Marcos; González Domínguez, José M.; Hernández Ferrer, Javier; et al.; Electrochemical sensing of guanine, adenine and 8-hydroxy-2′-deoxyguanosine at glassy carbon modified with single-walled carbon nanotubes covalently functionalized with lysine; Royal Society of Chemistry; RSC Advances; 6; 16; 21-1-2016; 13469-13477
2046-2069
CONICET Digital
CONICET
Autor
Gutierrez, Alejandro
Gutierrez, Fabiana Andrea
Eguílaz Rubio, Marcos
González Domínguez, José M.
Hernández Ferrer, Javier
Ansón Casaos, Alejandro
Martínez, María T.
Rivas, Gustavo Adolfo
Resumen
This work reports the synthesis and characterization of single-walled carbon nanotubes (SWCNT) covalently functionalized with l-lysine (Lys) and the analytical performance of glassy carbon electrodes (GCE) modified with a dispersion of SWCNT-Lys (GCE/SWCNT-Lys) for the highly sensitive quantification of guanine, adenine and 8-hydroxy-2′-deoxyguanosine. Detection limits of 75, 195 and 97 nM were obtained for guanine, adenine and 8-hydroxy-2′-deoxyguanosine, respectively by voltammetric adsorptive stripping with medium exchange. GCE/SWCNT-Lys was successfully used for the detection of adenine and guanine oxidation after adsorption of salmon sperm-double stranded DNA. A clear definition of 8-hydroxy-2′-deoxyguanosine oxidation signal is observed even in the presence of large excess of guanine, adenine or salmon sperm-double stranded DNA.