Artículos de revistas
Chemometric resolution of fully overlapped capillary electrophoresis peaks: quantitation of carbamazepine in human serum in the presence of several interferences
Fecha
2008-12Registro en:
Vera Candioti, Luciana; Culzoni, Maria Julia; Olivieri, Alejandro Cesar; Goicoechea, Hector Casimiro; Chemometric resolution of fully overlapped capillary electrophoresis peaks: quantitation of carbamazepine in human serum in the presence of several interferences; Wiley VCH Verlag; Electrophoresis; 29; 22; 12-2008; 4527-4537
0173-0835
CONICET Digital
CONICET
Autor
Vera Candioti, Luciana
Culzoni, Maria Julia
Olivieri, Alejandro Cesar
Goicoechea, Hector Casimiro
Resumen
Drug monitoring in serum samples was performed using second-order data generated by CE-DAD, processed with a suitable chemometric strategy. Carbamazepine could be accurately quantitated in the presence of its main metabolite (carbamazepine epoxide),other therapeutic drugs (lamotrigine, phenobarbital, phenytoin, phenylephrine,ibuprofen, acetaminophen, theophylline, caffeine, acetyl salicylic acid), and additional<br />serum endogenous components. The analytical strategy consisted of the following steps:(i) serum sample clean-up to remove matrix interferences, (ii) data pre-processing,in order to reduce the background and to correct for electrophoretic time shifts, and (iii) resolution of fully overlapped CE peaks (corresponding to carbamazepine, its metabolite, lamotrigine and unexpected serum components) by the well-known multivariate curve resolution-alternating least squares algorithm, which extracts quantitative information that can be uniquely ascribed to the analyte of interest. The analyte concentration in serum samples ranged from 2.00 to 8.00 mg/L. Mean recoveries<br />were 102.6% (s57.7) for binary samples, and 94.8% (s513.5) for spiked serum samples,while CV (%)54.0 was computed for five replicate, indicative of the acceptable accuracy and precision of the proposed method