info:eu-repo/semantics/article
Overlapping local and long-range RNA-RNA interactions modulate dengue virus genome cyclization and replication
Fecha
2015-03Registro en:
de Borba, Luana; Villordo, Sergio; Iglesias, Nestor Gabriel; Filomatori, Claudia Veronica; Gebhard, Leopoldo German; et al.; Overlapping local and long-range RNA-RNA interactions modulate dengue virus genome cyclization and replication; American Society For Microbiology; Journal Of Virology; 89; 6; 3-2015; 3430-3437
0022-538X
1098-5514
CONICET Digital
CONICET
Autor
de Borba, Luana
Villordo, Sergio
Iglesias, Nestor Gabriel
Filomatori, Claudia Veronica
Gebhard, Leopoldo German
Gamarnik, Andrea Vanesa
Resumen
The dengue virus genome is a dynamic molecule that adopts different conformations in the infected cell. Here, using RNA folding predictions, chemical probing analysis, RNA binding assays, and functional studies, we identified new cis-acting elements present in the capsid coding sequence that facilitate cyclization of the viral RNA by hybridization with a sequence involved in a local dumbbell structure at the viral 3' untranslated region (UTR). The identified interaction differentially enhances viral replication in mosquito and mammalian cells.