info:eu-repo/semantics/article
A new method for the preparation of biocompatible silica coated-collagen hydrogels
Fecha
2013-09Registro en:
Diaz, Luis Eduardo; Desimone, Martín Federico; Foglia, María Lucía; Camporotondi, Daniela Edhit; Alvarez, Gisela Solange; et al.; A new method for the preparation of biocompatible silica coated-collagen hydrogels; Royal Society of Chemistry; Journal of Materials Chemistry B; 1; 45; 9-2013; 6283-6290
2050-750X
Autor
Diaz, Luis Eduardo
Desimone, Martín Federico
Foglia, María Lucía
Camporotondi, Daniela Edhit
Alvarez, Gisela Solange
Heinemann, Sascha
Hanke, Thomas
Pérez, Claudio Javier
Resumen
Silica-collagen scaffolds were obtained by covalent binding of an aminosilane to glutaraldehyde fixed collagen hydrogels, rendering a three dimensional network of silicon coated collagen fibrils. When compared to non silicified collagen, silica containing matrices exhibited a 60 fold increment in the rheological properties. Moreover, acellular degradation collagenase type I indicated that enzymatic digestion occurred at a slower rate for silica modified hydrogels, hence enabling a controlled degradation of the obtained material. In addition, fibroblastic cells seeded on silicified collagen matrices were able to adhere, proliferate and migrate within the scaffold for over 3 weeks as shown by MTT tests and hematoxylin-eosin staining. These results suggest that the herein described method could be useful in the design of materials for tissue engineering purposes