Artículos de revistas
Development of a cell line from Echinococcus granulosus germinal layer
Fecha
2013-07Registro en:
Albani, Clara Maria; Cumino, Andrea Carina; Elissondo, María Celina; Denegri, Guillermo Maria; Development of a cell line from Echinococcus granulosus germinal layer; Elsevier Science; Acta Tropica; 128; 1; 7-2013; 124-129
0001-706X
CONICET Digital
CONICET
Autor
Albani, Clara Maria
Cumino, Andrea Carina
Elissondo, María Celina
Denegri, Guillermo Maria
Resumen
In vitro culture of parasitic helminths provides an important tool to study cell regeneration and physiology, as well as for molecular biology and genetic engineering studies. In the present study, we established in vitro propagation of cells from Echinococcus granulosus germinal cyst layer. E. granulosus germinal cells grew beyond 100 passages and showed no signs of reduced proliferation capacity. Microscopic analysis revealed that cells grew both attached to the substrate and in suspension, forming three-dimensional structures like mammalian stem cell aggregates. Examination of the chromosome number of attached germinal cells showed a high degree of heteroploidy, suggesting the occurrence of transformation during culture. Monolayer cells survived cryopreservation and were able to proliferate after thawing. Based on the characteristics displayed by E. granulosus germinal cells, we establish a cell line from the E. granulosus germinal layer. Furthermore, we propose that this cell line could be useful for drug screening and for obtaining parasite material.