Artículos de revistas
Novel tnpR-based transposable promoter traps suitable for RIVET studies in different gram-negative bacteria
Fecha
2013-02Registro en:
Lozano, Mauricio Javier; Salas, María Eugenia; Giusti, María de Los Ángeles; Martini, María Carla; López, José Luis; et al.; Novel tnpR-based transposable promoter traps suitable for RIVET studies in different gram-negative bacteria; Elsevier Science; Journal Of Microbiological Methods; 93; 1; 2-2013; 9-11
0167-7012
CONICET Digital
CONICET
Autor
Lozano, Mauricio Javier
Salas, María Eugenia
Giusti, María de Los Ángeles
Martini, María Carla
López, José Luis
Salto, Ileana Paula
del Papa, Maria Florencia
Pistorio, Mariano
Lagares, Antonio
Resumen
The preparation of plasmid-borne RIVET libraries can be troublesome when high genomic coverages are needed. We present here the construction and functional validation of a new set of miniTn5 promoter traps to generate tnpR-based RIVET libraries. The ability to generate tnpR transcriptional fusions by transposition will significantly facilitate the setup of RIVET studies in those bacteria where Tn5 transposition is operative.