info:eu-repo/semantics/article
Unlipidated Outer Membrane Protein Omp16 (U-Omp16) from Brucella spp. as Nasal Adjuvant Induces a Th1 Immune Response and Modulates the Th2 Allergic Response to Cow's Milk Proteins
Fecha
2013-07Registro en:
Ibañez, Andres Esteban; Smaldini, Paola Lorena; Coria, Lorena; Delpino, María Victoria; Pacífico, Lucila G.; et al.; Unlipidated Outer Membrane Protein Omp16 (U-Omp16) from Brucella spp. as Nasal Adjuvant Induces a Th1 Immune Response and Modulates the Th2 Allergic Response to Cow's Milk Proteins; Public Library Science; Plos One; 8; 7; 7-2013; 1-13
1932-6203
Autor
Ibañez, Andres Esteban
Smaldini, Paola Lorena
Coria, Lorena
Delpino, María Victoria
Pacífico, Lucila G.
Oliveira, Sergio C.
Risso, Gabriela Sofía
Pasquevich, Karina Alejandra
Fossati, Carlos Alberto
Giambartolomei, Guillermo Hernan
Docena, Guillermo H.
Cassataro, Juliana
Resumen
The discovery of novel mucosal adjuvants will help to develop new formulations to control infectious and allergic diseases. In this work we demonstrate that U-Omp16 from Brucella spp. delivered by the nasal route (i.n.) induced an inflammatory immune response in bronchoalveolar lavage (BAL) and lung tissues. Nasal co-administration of U-Omp16 with the model antigen (Ag) ovalbumin (OVA) increased the amount of Ag in lung tissues and induced OVA-specific systemic IgG and T helper (Th) 1 immune responses. The usefulness of U-Omp16 was also assessed in a mouse model of food allergy. U-Omp16 i.n. administration during sensitization ameliorated the hypersensitivity responses of sensitized mice upon oral exposure to Cow's Milk Protein (CMP), decreased clinical signs, reduced anti-CMP IgE serum antibodies and modulated the Th2 response in favor of Th1 immunity. Thus, U-Omp16 could be used as a broad Th1 mucosal adjuvant for different Ag formulations. © 2013 Ibañez et al.