dc.creator | Rosso, Marina | |
dc.creator | Lapyckyj, Lara | |
dc.creator | Amiano, Nicolás Oscar | |
dc.creator | Besso, María José | |
dc.creator | Sanchez, Mercedes Leonor | |
dc.creator | Chuluyan, Hector Eduardo | |
dc.creator | Vazquez, Monica Hebe | |
dc.date.accessioned | 2017-03-22T14:35:33Z | |
dc.date.accessioned | 2018-11-06T11:32:28Z | |
dc.date.available | 2017-03-22T14:35:33Z | |
dc.date.available | 2018-11-06T11:32:28Z | |
dc.date.created | 2017-03-22T14:35:33Z | |
dc.date.issued | 2014-09 | |
dc.identifier | Rosso, Marina; Lapyckyj, Lara; Amiano, Nicolás Oscar; Besso, María José; Sanchez, Mercedes Leonor; et al.; Secretory leukocyte protease inhibitor (slpi) expression downregulates E-cadherin, induces β-catenin re-localization and triggers apoptosis-related events in breast cancer cells; Wiley; Biology Of The Cell; 106; 9; 9-2014; 308-322 | |
dc.identifier | 0248-4900 | |
dc.identifier | http://hdl.handle.net/11336/14168 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1854448 | |
dc.description.abstract | BACKGROUND INFORMATION: Epithelial cadherin (E-cadherin) is involved in cell–cell adhesion through its extracellular domain, whereas the intracellular domain interacts with adaptor proteins, i.e. β-catenin, links E-cadherin to the actin cytoskeleton and participates in signal transduction events. E-cadherin protects mammary epithelial cells from apoptosis and its loss during tumour progression has been documented. Secretory Leukocyte Protease Inhibitor (SLPI) has anti- and pro-tumourigenic activities but its role in breast cancer has not been fully elucidated. Notwithstanding its relevance, how SLPI affects E-cadherin in breast cancer is still unknown. This study evaluated the effect of SLPI upon E-cadherin/β-catenin expression and apoptosis-related markers in murine (F3II) and human (MCF-7) breast tumour cells either treated with exogenous recombinant human SLPI (rhSLPI) or stably transfected with a plasmid encoding its sequence. RESULTS: Addition of rhSLPI to F3II cells caused a decrease (P < 0.05) in E-cadherin transcript and protein levels. Similar results were observed in SLPI-stable F3II transfectants (2C1), and treatment of 2C1 cells with a siRNA toward SLPI restored E-cadherin to control levels. SLPI-expressing cells showed disruption of E-cadherin/β-catenin complex and increased (P < 0.05) percentage of cells depicting nuclear β-catenin localisation. Associated to these changes, 2C1 cells showed increased Bax/Bcl-2 ratio and p21 protein levels, decreased c-Myc protein levels and decreased Cyclin D1 and Claudin-1 transcript levels. No differences in N- and P-cadherin were observed between SLPI-transfected cells and controls. Addition of rhSLPI to MCF-7 cells or stable transfection with SLPI caused a decrease (P < 0.05) in E-cadherin expression (transcript/protein) and its redistribution to the cytoplasm, as well as β-catenin re-localisation to the cell nucleus. CONCLUSIONS: Expression of SLPI was associated to a decrease in E-cadherin expression and re-localisation of E-cadherin to the cell cytoplasm and β-catenin to the cell cytoplasm and nucleus, and had pro-apoptotic and cell cycle-arrest effects. | |
dc.language | eng | |
dc.publisher | Wiley | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/boc.201300075/abstract | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/boc.201300075 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | APOPTOSIS | |
dc.subject | BETA-CATENINA | |
dc.subject | CANCER | |
dc.subject | CELL ADHESION | |
dc.subject | EPHITELIAL CADHERIN | |
dc.subject | SLPI | |
dc.title | Secretory leukocyte protease inhibitor (slpi) expression downregulates E-cadherin, induces β-catenin re-localization and triggers apoptosis-related events in breast cancer cells | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |