Artículos de revistas
The carboxy terminal domain of RNA polymerase II and alternative splicing
Muñoz, Manuel Javier; de la Mata, Manuel; Kornblihtt, Alberto Rodolfo; The carboxy terminal domain of RNA polymerase II and alternative splicing; Cell Press; Trends In Biochemical Sciences; 35; 9; 9-2010; 497-504
Muñoz, Manuel Javier
de la Mata, Manuel
Kornblihtt, Alberto Rodolfo
Alternative splicing is controlled by cis-regulatory sequences present in the pre-mRNA and their cognate trans-acting factors, as well as by its coupling to RNA polymerase II (pol II) transcription. A unique feature of this polymerase is the presence of a highly repetitive carboxy terminal domain (CTD), which is subject to multiple regulatory post-translational modifications. CTD phosphorylation events affect the transcriptional properties of pol II and the outcome of co-transcriptional alternative splicing by mediating the effects of splicing factors and by modulating transcription elongation rates. Here, we discuss various examples of involvement of the CTD in alternative splicing regulation as well as the current methodological limitations in deciphering the detailed mechanisms of this process.