dc.creator | Durán, Gema M. | |
dc.creator | Benavidez, Tomás Enrique | |
dc.creator | Ríos, Ángel | |
dc.creator | Garcia, Carlos D. | |
dc.date.accessioned | 2018-08-10T17:38:19Z | |
dc.date.accessioned | 2018-11-06T11:27:39Z | |
dc.date.available | 2018-08-10T17:38:19Z | |
dc.date.available | 2018-11-06T11:27:39Z | |
dc.date.created | 2018-08-10T17:38:19Z | |
dc.date.issued | 2016-02 | |
dc.identifier | Durán, Gema M.; Benavidez, Tomás Enrique; Ríos, Ángel; Garcia, Carlos D.; Quantum dot-modified paper-based assay for glucose screening; Springer Wien; Mikrochimica Acta; 183; 2; 2-2016; 611-616 | |
dc.identifier | 0026-3672 | |
dc.identifier | http://hdl.handle.net/11336/54967 | |
dc.identifier | 1436-5073 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1852352 | |
dc.description.abstract | The article describes a simple optical assay for glucose. It is based on the use of paper spots loaded with colloidal CdSe/ZnS quantum dots (Q-dots) and the enzyme glucose oxidase (GOx). Circular paper sheets were uniformly loaded with Q-dots and then displayed strong fluorescence under a UV lamp (365 nm exCitation). The action of GOx causes the production of H2O2 which, after a typical exposure time of 20 min, causes fluorescence intensity to be quenched. To obtain a reading, the paper sheets were photographed under 365 nm excitation using a digital camera. Several parameters, including the amount of Q-dots, sample pH, and amount of GOx were optimized to maximize the response to glucose. The paper-based assay showed a sigmoidal-shaped response with respect to the glucose concentration in the 5–200 mg·dL−1 range (limit of detection of 5 μg·dL−1), demonstrating their potential use for biomedical applications. | |
dc.language | eng | |
dc.publisher | Springer Wien | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://link.springer.com/article/10.1007%2Fs00604-015-1685-3 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00604-015-1685-3 | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4820292/ | |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | GLUCOSE | |
dc.subject | PAPER-BASED ASSAY | |
dc.subject | QUANTUM DOTS | |
dc.title | Quantum dot-modified paper-based assay for glucose screening | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |