Artículos de revistas
Quantum dot-modified paper-based assay for glucose screening
Fecha
2016-02Registro en:
Durán, Gema M.; Benavidez, Tomás Enrique; Ríos, Ángel; Garcia, Carlos D.; Quantum dot-modified paper-based assay for glucose screening; Springer Wien; Mikrochimica Acta; 183; 2; 2-2016; 611-616
0026-3672
1436-5073
CONICET Digital
CONICET
Autor
Durán, Gema M.
Benavidez, Tomás Enrique
Ríos, Ángel
Garcia, Carlos D.
Resumen
The article describes a simple optical assay for glucose. It is based on the use of paper spots loaded with colloidal CdSe/ZnS quantum dots (Q-dots) and the enzyme glucose oxidase (GOx). Circular paper sheets were uniformly loaded with Q-dots and then displayed strong fluorescence under a UV lamp (365 nm exCitation). The action of GOx causes the production of H2O2 which, after a typical exposure time of 20 min, causes fluorescence intensity to be quenched. To obtain a reading, the paper sheets were photographed under 365 nm excitation using a digital camera. Several parameters, including the amount of Q-dots, sample pH, and amount of GOx were optimized to maximize the response to glucose. The paper-based assay showed a sigmoidal-shaped response with respect to the glucose concentration in the 5–200 mg·dL−1 range (limit of detection of 5 μg·dL−1), demonstrating their potential use for biomedical applications.