info:eu-repo/semantics/article
Confined Gold Nanoparticles Enhance the Detection of Small Molecules in Label-Free Impedance Aptasensors
Fecha
2015-03-19Registro en:
Peinetti, Ana Sol; Ceretti, Helena; Mizrahi, Martin Daniel; González, Graciela Alicia; Ramirez, Silvana Andrea Maria; et al.; Confined Gold Nanoparticles Enhance the Detection of Small Molecules in Label-Free Impedance Aptasensors; Royal Society of Chemistry; Nanoscale; 7; 17; 19-3-2015; 7763-7769
2040-3364
Autor
Peinetti, Ana Sol
Ceretti, Helena
Mizrahi, Martin Daniel
González, Graciela Alicia
Ramirez, Silvana Andrea Maria
Requejo, Felix Gregorio
Montserrat, Javier Marcelo
Battaglini, Fernando
Resumen
A controlled architecture of nanoelectrodes, of a similar size to small molecule-binding aptamers, is synthesized inside nanoporous alumina. Gold nanoparticles with a controlled size (about 2 nm) are electrogenerated in the alumina cavities, showing a fast electron transfer process toward ferrocyanide. These uncapped nanoparticles are easily modified with a thiol-containing aptamer for label-free detection of adenosine monophosphate by electrochemical impedance spectroscopy. Our results show that the use of a limited electrical conducting surface inside an insulating environment can be very sensitive to conformational changes, introducing a new approach to the detection of small molecules, exemplified here by the direct and selective detection of adenosine monophosphate at the nanomolar scale.