info:eu-repo/semantics/article
Immunohistochemical distribution of early pregnancy factor in ovary, oviduct and placenta of pregnant gilts
Registro en:
Grosso, Maria Carolina; Bellingeri, Romina Valeria; Motta, C; Alustiza, Fabrisio Eduardo; Picco, Natalia Yanina; et al.; Immunohistochemical distribution of early pregnancy factor in ovary, oviduct and placenta of pregnant gilts; Informa Healthcare; Biotechnic And Histochemistry; 90; 1; 6-2014; 14-24
1052-0295
CONICET Digital
CONICET
Autor
Grosso, Maria Carolina
Bellingeri, Romina Valeria
Motta, C
Alustiza, Fabrisio Eduardo
Picco, Natalia Yanina
Vivas, Adriana Beatriz
Resumen
Early pregnancy factor (EPF) is an immunosuppressant that promotes maternal immune system tolerance of the allogenic fetus. Little is known about localization of this factor in different tissues and nothing has been reported about localization in swine reproductive and placental tissues. We determined the concentration of EPF in serum of gilts and porcine placenta conditioned medium (PPCM). We also analyzed the expression of EPF in different reproductive tissues of pregnant gilts at 10, 30, 60 and 90 days of pregnancy. EPF concentration in serum and PPCM was determined by western blot and densitometry. EPF expression in reproductive tissue was assessed by immunohistochemistry. The highest concentration of EPF was observed at 30 days in serum and PPCM; the concentration was higher in PPCM than in serum at the stages we evaluated. All reproductive tissues from the gestational stages analyzed showed specific labeling of EPF, but this labeling did not appear in non-pregnant gilts. At 30 days pregnancy, the EPF expression in the ovary was predominantly in follicular lutein cells, probably owing to its function as a luteotrophic factor. In the oviduct, EPF was expressed in unciliated secretory epithelial cells and in the cilia of ciliated cells. In the placenta, EPF was expressed in the fetal portion (mesoderm chorioallantois and epithelium of endoderm). EPF acts as an autocrine and paracrine growth factor for the trophoblast during the peri-implantation period. Fil: Grosso, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina Fil: Bellingeri, Romina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina Fil: Motta, C. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina Fil: Alustiza, Fabrisio Eduardo. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina Fil: Picco, Natalia Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina Fil: Vivas, Adriana Beatriz. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Anatomia Animal; Argentina