Artículos de revistas
Benznidazole levels in blood vary with age in rats
Fecha
2011-05Registro en:
Bulffer, Romina Fernanda; Castro, Jose Alberto; Fanelli, Silvia Laura; Benznidazole levels in blood vary with age in rats; Fundacao Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 106; 3; 5-2011; 374-377
0074-0276
1678-8060
CONICET Digital
CONICET
Autor
Bulffer, Romina Fernanda
Castro, Jose Alberto
Fanelli, Silvia Laura
Resumen
Benznidazole (Bz) exhibits toxic side effects in animal studies and clinical use. Reductive metabolism of Bz in liver microsomes modulates the duration of its chemotherapeutic effect and its toxicity. The rate of this metabolism depends on age and is less intense in newborns and youngsters than in adults. In the present study, we determined Bz blood levels in rats of different ages that received Bz intragastrically (100 mg/kg). We developed and validated a high-pressure liquid chromatography with UV detector method for determination of Bz levels in whole blood. Bz levels were significantly higher and persisted for longer periods of time in the blood of young rats when compared to that of adult animals.