info:eu-repo/semantics/article
A highly sensitive rapid diagnostic test for Chagas disease that utilizes a recombinant Trypanosoma cruzi antigen
Registro en:
Barfield, C. A.; Barney, R. S.; Crudder, C. H.; Wilmoth, J. L.; Stevens; D. S.; et al.; A highly sensitive rapid diagnostic test for Chagas disease that utilizes a recombinant Trypanosoma cruzi antigen; Institute Of Electrical And Electronics Engineers; Ieee Transactions On Bio-medical Engineering; 58; 3; 3-2011; 814-817
0018-9294
Autor
Barfield, C. A.
Barney, R. S.
Crudder, C. H.
Wilmoth, J. L.
Stevens; D. S.
Mora Garcia, Santiago
Yanovsky, Marcelo Javier
Weigl, B. H.
Yanovsky, J.
Resumen
Improved diagnostic tests for Chagas disease are urgently needed. A new lateral flow rapid test for Chagas disease is under development at PATH, in collaboration with Laboratorio Lemos of Argentina, which utilizes a recombinant antigen for detection of antibodies to Trypanosoma cruzi. To evaluate the performance of this test, 375 earlier characterized serum specimens from a region where Chagas is endemic were tested using a reference test (the Ortho T. cruzi ELISA, Johnson & Johnson), a commercially available rapid test (Chagas STAT-PAK, Chembio), and the PATH-Lemos rapid test. Compared to the composite reference tests, the PATH-Lemos rapid test demonstrated an optimal sensitivity of 99.5% and specificity of 96.8%, while the Chagas STAT-PAK demonstrated a sensitivity of 95.3% and specificity of 99.5%. These results indicate that the PATH-Lemos rapid test shows promise as an improved and reliable tool for screening and diagnosis of Chagas disease. Fil: Barfield, C. A.. PATH; Estados Unidos Fil: Barney, R. S.. PATH; Estados Unidos Fil: Crudder, C. H.. PATH; Estados Unidos Fil: Wilmoth, J. L.. PATH; Estados Unidos Fil: Stevens; D. S.. PATH; Estados Unidos Fil: Mora Garcia, Santiago. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Yanovsky, Marcelo Javier. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Weigl, B. H.. PATH; Estados Unidos Fil: Yanovsky, J.. Laboratorio Lemos; Argentina