Effect of phytase supplementation of a plant-based diet on phosphorus and nitrogen bioavailability in sea bream Sparus aurata

dc.creatorMorales, Gabriel Alejandro
dc.creatorMarquez, L.
dc.creatorSaenz de Rodrigañez, M.
dc.creatorBermúdez, L.
dc.creatorRobles, R.
dc.creatorMoyano, F. J.
dc.date2014-04
dc.identifierhttp://hdl.handle.net/11336/16353
dc.identifierMorales, Gabriel Alejandro; Marquez, L.; Saenz de Rodrigañez, M.; Bermúdez, L.; Robles, R.; et al.; Effect of phytase supplementation of a plant-based diet on phosphorus and nitrogen bioavailability in sea bream Sparus aurata; Wiley; Aquaculture Nutrition; 20; 2; 4-2014; 172-182
dc.identifier1353-5773
dc.identifier1365-2095
dc.descriptionTo assess to what extent addition of phytase to a plant-based diet results in spatio-temporal changes of phytate, available P, soluble protein, total amino acids and the activity of the main digestive proteases in gilthead sea bream, fish were fed two plant-based diets with or without phytase. Stomach, proximal intestine and distal intestine contents were monitored for these parameters at 0, 1, 2, 4 and 6 h after feeding. A reduction (P < 0.0001) of the soluble P–IP6 in the stomach when phytase was added to the diet was observed. Within stomach, most of the total P–IP6 was precipitated (86%), possibly due to the low acidification capacity of the sea bream (pH > 4), but 57% of the dietary P–IP6 was dephosphorylated, suggesting that phytase could have the capacity to dephosphorylate insoluble IP6 at such pH. An increment (60%) (P < 0.01) in total gastric protease activity was observed by phytase addition, this being the first demonstration of the in vivo effect of IP6 on the pepsin activity in fish stomach. Gastric pH and residence time of the digesta inside the stomach are critical factors for an efficient phytase action and improve P and N bioavailability in plant-based diets used in fish aquaculture.
dc.descriptionFil: Morales, Gabriel Alejandro. Universidad de Almería; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.descriptionFil: Marquez, L.. Universidad de Almería; España
dc.descriptionFil: Saenz de Rodrigañez, M.. Universidad de Almería; España
dc.descriptionFil: Bermúdez, L.. Fundación Centro Tecnológico de Acuicultura de Andalucía; España
dc.descriptionFil: Robles, R.. Fundación Centro Tecnológico de Acuicultura de Andalucía; España
dc.descriptionFil: Moyano, F. J.. Universidad de Almería; España
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherWiley
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/10.1111/anu.12063
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/anu.12063/abstract
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subjectAmino Acids
dc.subjectDigestive Proteases
dc.subjectPhosphorus
dc.subjectPhytase
dc.subjectPhytate
dc.subjectSparus Aurata
dc.subjecthttps://purl.org/becyt/ford/4.2
dc.subjecthttps://purl.org/becyt/ford/4
dc.titleEffect of phytase supplementation of a plant-based diet on phosphorus and nitrogen bioavailability in sea bream Sparus aurata
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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