Spatiotemporal properties of high-speed calcium oscillations in the pedunculopontine nucleus

dc.creatorHyde, James
dc.creatorKezunovic, Nebojsa
dc.creatorUrbano Suarez, Francisco Jose
dc.creatorGarcia Rill, Edgar
dc.date2013-11
dc.identifierhttp://hdl.handle.net/11336/21101
dc.identifierHyde, James; Kezunovic, Nebojsa; Urbano Suarez, Francisco Jose; Garcia Rill, Edgar; Spatiotemporal properties of high-speed calcium oscillations in the pedunculopontine nucleus; American Physiological Society; Journal Of Applied Physiology; 115; 9; 11-2013; 1402-1414
dc.identifier8750-7587
dc.identifier1522-1601
dc.identifierCONICET Digital
dc.identifierCONICET
dc.descriptionThe pedunculopontine nucleus (PPN) is a component of the reticular activating system (RAS), and is involved in the activated states of waking and rapid eye movement (REM) sleep. Gamma oscillations (approximately 30–80 Hz) are evident in all PPN neurons and are mediated by high-threshold voltage-dependent N- and P/Q-type calcium channels. We tested the hypothesis that high-speed calcium imaging would reveal calcium-mediated oscillations in dendritic compartments in synchrony with patch-clamp recorded oscillations during depolarizing current ramps. Patch-clamped 8- to 16-day-old rat PPN neurons (n = 67 out of 121) were filled with Fura 2, Bis Fura, or OGB1/CHR. This study also characterized a novel ratiometric technique using Oregon Green BAPTA-1 (OGB1) with coinjections of a new long-stokes-shift dye, Chromeo 494 (CHR). Fluorescent calcium transients were blocked with the nonspecific calcium channel blocker cadmium, or by the combination of ω-agatoxin-IVA, a specific P/Q-type calcium channel blocker, and ω-conotoxin-GVIA, a specific N-type calcium channel blocker. The calcium transients were evident in different dendrites (suggesting channels are present throughout the dendritic tree) along the sampled length without interruption (suggesting channels are evenly distributed), and appeared to represent a summation of oscillations present in the soma. We confirm that PPN calcium channel-mediated oscillations are due to P/Q- and N-type channels, and reveal that these channels are distributed along the dendrites of PPN cells.
dc.descriptionFil: Hyde, James. University Of Arkansas For Medical Sciences; Estados Unidos
dc.descriptionFil: Kezunovic, Nebojsa. University Of Arkansas For Medical Sciences; Estados Unidos
dc.descriptionFil: Urbano Suarez, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
dc.descriptionFil: Garcia Rill, Edgar. University Of Arkansas For Medical Sciences; Estados Unidos
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherAmerican Physiological Society
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/10.1152/japplphysiol.00762.2013
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://jap.physiology.org/content/115/9/1402
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subjectArousal
dc.subjectPedunculopontine
dc.subjectCalcium Imaging
dc.subjectP/Q Type Calcium Channels
dc.subjecthttps://purl.org/becyt/ford/3.1
dc.subjecthttps://purl.org/becyt/ford/3
dc.titleSpatiotemporal properties of high-speed calcium oscillations in the pedunculopontine nucleus
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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